By Alan A. Boulton, Glen B. Baker, Judith M. Baker
Strategies u-r the neurosciences are evolving speedily. There are at present only a few volumes devoted to the technique - ployed by way of neuroscrentists, and those who can be found frequently appear both outdated or restricted in scope This sequence is ready the equipment most generally utilized by modern day neuroscrentrsts and 1s written via their colleagues who're working towards specialists quantity 1 might be priceless to all neuroscientists because it issues these tactics used usually around the widest diversity of subdiscrplines Collectmg those normal techmques jointly in one quantity moves us not just as a carrier, yet will without doubt turn out of exceptronal utilrtarian worth in addition Volumes 2 and three - scribe all present techniques for the analyses of ammes and their metabolrtes and of ammo acids, respectively. those collectrons will basically be of price to all neuroscrentrsts operating m or considering examine in these fields. comparable purposes exist for quantity four on receptor bmdmg thoughts on account that experimental - tails are supplied for all sorts of lrgand-receptor binding, together with chapters on basic rules, drug discovery and - velopment, and a most beneficial appendix on desktop courses for Scatchard, nonlinear, and competitrve displacement analyses. quantity five offers methods for the review of enzymes- volved m brogemc amme synthesis and catabolrsm. Volumes within the NEUROMETHODS sequence may be invaluable to neurochemists, -pharmacologists, -physrologrsts, -anatomrsts, psychopharmacologrsts, psychratrrsts, neurologrsts, and chemists (organic, analytrcal, pharmaceutrcal, medicinal), m truth, all people concerned m the neurosciences, either simple and medical.
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Additional info for Amines and Their Metabolites
Each homogenate is frozen in liquid nltrogen and stored at 4°C until homogenization of all samples has been completed. 12 mL) is added to each. After addition of excess solid potassium chloride, samples are centrifuged at 0°C (lS,OOO~, 5 mm) to precipitate protein and remove the potassium perchlorate precipitate. Supernatants are transferred to glass-stoppered tubes, a few crystals of potassium chloride are added to ensure saturation, and ti-butyl acetate (10 mL) IS added to each tube The tubes are shaken by hand (5 mm) and centrifuged (1 min) at room temperature.
N 1 Pharmacol 25, 727-736. Kariya T and Apnson M H. (1969) Mlcrodetermmatlon of norepmephrme, 3,4-dlhydroxyphenylethylamme, and 5-hydroxytryptamme from single extracts of specific rat brain areas Anal Blochem 31, 102-113. Kehr W (1974) A method for the lsolatlon and determmatlon of 3-methoxytyramme m brain tissue. Naunyn-Schmled Arch Pharmacol 284, 149-158 Korf J and Valkenburgh-Slkkema T. (1969) Fluorlmetrlc determination of 5-hydroxymdoleacetlc acid in human urine and cerebrospmal fIuld Clm Chrm Actfi 26, 301-306 Kostyukovskn Ya L and Melamed D B.
Contractor S. F. (1966) A rapid quantitative method for the estimation of 5-hydroxyindoleacetic acid m human urine Blochem. Pharmacol. 15, 1701-1706. Cox R H. Jr. and Perhach J L Jr (1973) A sensitive, rapid, and simple method for the simultaneous spectrophotofluorometric determma- Baker, Butterworth, 34 and Dewhurst non of norepmephrme, dopamme, 5-hydroxytryptamme, and 5-hydroxymdoleacetic acid m discrete areas of brain. ] Neurockem 20, 1777-1780. Crawford T B B. and Yates C M. (1970) A method for the estimation of the catecholammes and their metabohtes m brain tissue.
Amines and Their Metabolites by Alan A. Boulton, Glen B. Baker, Judith M. Baker